P-038 Correlation between sperm DNA fragmentation index, semen parameters and Human Papillomavirus: an analysis conducted under World Health Organization 2021 guidelines

Abstract Study question Following 6thWHO (2021), we analysed the correlation between DNA fragmentation index (DFI), Human papillomavirus (HPV), and seminal parameters, highlighting slow rapid progressive motility alterations. Summary answer DFI rates parameters correlated with rapid, slow, motility. However, HPV-positivity caused loss of association What is known already HPV detection in semen samples has long opened an investigation into its influence on male infertility. Some studies indicate that can affect sperm quality DFI, while others have failed to find any correlation. With reference 2010 WHO guidelines, our latest work highlighted how positivity significantly impairs motility, morphology, immotile rate. Since 2021 guidelines included evaluation if these new other conventional could be altered by infection. design, size, duration From August December 2022, 121 were collected from partners HPV-positive women attending vitro fertilization (IVF). Every specimen underwent evaluation, analysis test. Participants/materials, setting, methods Seminal masturbation after 3-5 days sexual abstinence. The inclusion criteria as follows: no sexually transmitted infections, genetic diseases, inflammatory disorders. Sperm concentration, non-progressive sperms, both evaluated according guidelines. was assessed chromatin dispersion test (SCD), HPV-DNA performed using InnoLipa Genotyping Extra II (Fujirebio, Tokyo, Japan). Main results role chance Of tested, 60 (49.6%) 61 (50.4%) HPV-negative. showed a significant negative groups positive HPV-negative group. Conversely, significance completely lost patients. normal forms spermatozoa percentages Similar observed samples, except for form rate, which not associated In addition, same displayed absent samples. Significant associations found also derived parameter sperm, rate groups. suggest high rates, presence or absence infection, reproductive health through consistent impairment particular, distinction allows deeper understanding possible correlations Limitations, reasons caution This preliminary study characterized small number Therefore, confirmation findings requires enlargement patient cohort, taking place. Wider implications Our highlight introduction criteria, i.e. provides additional information about impact infection it. Trial registration Not applicable